Figure 5.

Importance of the leucine and valine residues for the anterograde transport signal. The terminal two residues of the control reporter (WWS) were mutated as indicated at the bottom by the single-letter amino acid code. LV, WWL; SS, WWSS. Pulse–chase experiments were done, and transport rate constants were calculated as described for Figures 2 and 3. (A) Rate constants of transport to the vacuole. (B) Rate constants of ER to cis-Golgi transport. Bars, mean values of three experiments; error bars, SD. The values on top of the bars indicate the relative acceleration of the transport (%).