Figure 6.

Importance of the glutamine residues in the TMD. (A) Glutamine residues in the TMD of WES construct (QQ) were mutated to alanines. The positions of the mutated residues are indicated (A) underneath the original Emp24p transmembrane sequence. The names of the constructs are indicated on the left. (B) Rate constants of transport to the vacuole. (C) Rate constants of ER to cis-Golgi transport. Pulse–chase experiments were done, and transport rate constants were calculated as described for Figures 2 and 3. Bars, mean values; error bars, SD. The numbers of experiments are shown on top of the bars.