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Descriptive statistics of Acanthamoeba DNA detection by real-time PCR
| Parameter | Assay
|
|
|---|---|---|
| Rivière | Qvarnstrom | |
| Lowest dilution (plasmid DNA) | 10−11 | 10−9 |
| LOD (mean no. of copies/10 μl) | 11.3 | 43.8 |
| LOD (mass [ag]) | 38.12 | 157.75 |
| Correlation coefficient r2 (%) | 99.8 | 99.2 |
| AE (%) | 94 | 92 |
| LODa (mean no. of trophozoites/10 μl) | 2.3 | 2.3 |
| LODa (mean no. of cysts/10 μl) | 0.7 | 0.7 |
Data represent the results of two experiments.
