TABLE 4.

Comparison of two real-time PCR assays for the detection of Acanthamoeba DNA^a

Rivière real-time PCR result	Qvarnstrom real-time PCR result (no. of isolates)
	Positive	Negative	Indeterminate	Inhibitory	Total
Positive	11b	2c	0	0	13
Negative	0	149d	0	0	149
Indeterminate	0	3e	1f	0	4
Inhibitory	0	0	0	2g	2
Total	11	154	1	2	168

^a“Indeterminate” indicates a sample that tested positive initially, but the result was not reproducible. “Inhibitory” indicates samples where the internal control tested negative after extraction and re-extraction.

^bNine cornea and two CL cases. Seven were culture positive, real-time PCR positive, and four were culture negative, real-time PCR positive.

^cOne cornea, one CL solution bottle.

^d95 cornea, 32 CL cases, 20 CL solution bottles, 1 eyelid, 1 conjunctiva and eyelid.

^eTwo cornea, one CL case.

^fOne CL case.

^gTwo CL cases.