TABLE 3.
Comparison between molecular epidemiological data generated over six consecutive 2-year intervals by IS6110 RFLP and MIRU-VNTR genotyping methods
| Method | Avg value (range)
|
|||||
|---|---|---|---|---|---|---|
| No. of strains | No. of clusters | % Clustering | % Pair-wise matching concordance | % Pair-wise mismatching concordance | % Concordance between unique strains | |
| IS6110 RFLP | 17.9 (7-20) | 6.1 (4-10) | 74.5 (55.4-86.7) | 100 | NAf | 100 |
| MIRU-VNTR locus combinations | ||||||
| 12-MIRUa | 9.9 (5-14) | 4.6 (2-7) | 85.1 (60.0-93.1) | 69.7 (60-80) | 92.8 (72-99) | 19.1 (6.1-60.0) |
| 12-MIRU + ETR A, B, Cb | 10.9 (7-14) | 4.9 (3-7) | 83.1 (60.0-91.7) | 63.7 (44-72) | 95.0 (84-99) | 25.7 (15.6-60.0) |
| 12-MIRU + hypervariable locic | 15.4 (8-22) | 4.0 (2-6) | 73.1 (60.0-77.8) | 52.7 (40-58) | 94.0 (77-99) | 42.2 (27.0-66.7) |
| 15-MIRU-VNTRd | 12.0 (5-17) | 3.7 (2-5) | 80.3 (66.7-93.1) | 68.2 (45-78) | 92.3 (70-99) | 31.3 (16.7-60.0) |
| 15-MIRU-VNTR + hypervariable locie | 16.6 (7-23) | 3.6 (2-6) | 70.7 (57.6-76.4) | 51.0 (22-63) | 94.0 (76-100) | 50.9 (32.0-66.7) |
| 24-MIRU-VNTRd | 13.6 (7-19) | 4.6 (3-6) | 77.8 (60.0-89.7) | 55.0 (44-69) | 95.2 (84-99) | 35.4 (24.0-60.0) |
| 24-MIRU-VNTR + hypervariable locic | 18.1 (9-25) | 4.3 (3-7) | 67.7 (54.5-75.0) | 40.2 (22-51) | 95.0 (84-99) | 51.3 (32.0-66.7) |