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Specific growth inhibition of a P. aeruginosa cioAB mutant by the copper chelator TTM. The wild-type PAO1 and the cioAB mutant PAO6437 were grown in OS-glucose to an OD600 of approximately 2.5 and then 10 μl of each culture was spotted onto OS-glucose plates (A) supplemented with 1 mM TTM (B), with 1 mM TTM and 30 μM CuSO4 (C), or with 1 mM TTM and 300 μM CuSO4 (D). Incubation was at 37°C for 18 h.
