TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF)U169 [φ80dlacZΔΜ15] F− Nalr | 45 |
| HB101 | proA2 hsdS20(rB− m B−) recA13 ara-14 lacY1 galK2 rpsL20 supE44 xyl-5 mtl-1 F− | 45 |
| P. aeruginosa | ||
| PAO1 (ATCC 15692) | Wild type | American Type Culture Collection |
| PAO6437 | PAO1 containing a 2,400-bp deletion in the cioAB locus | This study |
| PAO6573 | PAO1 containing a 1,933-bp deletion in the copRS locus | This study |
| PAO6593 | PAO1 containing a 4,109-bp deletion in the coxBA-PA0107-coIII locus | This study |
| PAO6594 | PAO1 containing a 1,688-bp deletion in the roxSR locus | This study |
| PAO6597 | PAO6593 containing a 4,830-bp deletion in the cyoABCDE operon | This study |
| PAO6650 | PAO6597 containing a 6,445-bp deletion in the two adjacent ccoNOQP1 and ccoNOQP2 operons | This study |
| Plasmids | ||
| pRK2013 | Helper plasmid; Tra+ Kmr | 16 |
| pME3087 | Suicide vector for allelic replacement; Tcr; ColE1 replicon | 54 |
| pME3641 | Plasmid carrying a translational proC′-′lacZ fusion; Cbr | 46 |
| pME6013 | Cloning vector for translational lacZ fusions; Tcr | 47 |
| pME6015 | Cloning vector for translational lacZ fusions; Tcr | 47 |
| pME6016 | Cloning vector for transcriptional lacZ fusions; Tcr | 47 |
| pME6031 | Expression vector carrying ptac lacIQ; Tcr | 23 |
| pME7226 | Plasmid carrying a translational pchR′- ′lacZ fusion; Tcr | 34 |
| pME7541 | Suicide construct used for deletion of the cioAB operon; Tcr | This study |
| pME7554 | Plasmid carrying a translational cioA′- ′lacZ fusion; Tcr | This study |
| pME7576 | Suicide construct used for deletion of the copRS operon; Tcr | This study |
| pME9301 | Plasmid carrying a translational pvdS′- ′lacZ fusion; Tcr | This study |
| pME9302 | Suicide construct used for deletion of the coxB-coIII cluster; Tcr | This study |
| pME9303 | Suicide construct used for deletion of the cyoABCDE operon; Tcr | This study |
| pME9305 | pME6031 derivative carrying the cioAB genes; Tcr | This study |
| pME9306 | Plasmid carrying a transcriptional cioA-lacZ fusion; Tcr | This study |
| pME9307 | Suicide construct used for deletion of the roxSR operon; Tcr | This study |
| pME9308 | Suicide construct used for deletion of the two adjacent ccoNOQP operons; Tcr | This study |