FIG. 3.

Immunoblots of fractions from affinity chromatography of PBP2 applied to SltB1-Sepharose and Tris-Sepharose. Purified preparations of recombinant PBP2 (A) and the isolated non-PB module (B) were applied to either SltB1-Sepharose or a control Tris-Sepharose column. Conditions of the affinity chromatography and SDS-PAGE were as described in the legend to Fig. 2. Following electrophoresis, the recombinant proteins were detected by Western immunoblot analysis using an anti-six-His antibody as described in Materials and Methods. The positions of molecular mass markers (in kilodaltons) are indicated on the left, and the arrows denote the positions of PBP2 (top) and the non-PB module (bottom).