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Construction of a C. crescentus ΔspoT strain and a complemented ΔspoT strain. (A) Schematic representation of the spoT locus and the ΔspoT strain used to characterize SpoT function. (B) Construction of the ΔspoT-complementing vector pJL38 and the negative control vector, pJL37. (C) Schematic representation of the complemented ΔspoT strain, ΔspoT/pJL38, and the control strains harboring the empty vector ΔspoT/pJL37 and wild-type/pJL37.
