(A) Maps of pMV01. V. anguillarum DNA, containing the EmpA-processing protease (epp) gene and flanking sequences, that was cloned into pBluescript SKII+ (pMV07) and the adjacent hemolysin cluster 1 are shown. Genes coding for the following products are represented: phospholipase/lecithinase (plp), hemolysin (vah1), lactonizing lipase (llpA), lactonizing lipase activator (llpB), EmpA-processing protease (epp), putative glutamine amidotransferase (gat), putative exoprotease (expA), sensor protein (cpxA), transcriptional regulator (cpxR), putative periplasmic protein (orfP), thiol:disulfide interchange protein (tdi), and a putative homoserine kinase (hsk). Arrows indicate the direction of transcription. (B) Map of hemolysin cluster 1, containing a putative protease gene from V. cholerae O1 biovar El Tor strain N16961. Genes and the corresponding tag numbers coding for the following products are represented: lecithinase (lec), hemolysin (hlyA), chemotaxis transducer (hlyB), lipase accessory (lipAB), and putative metalloprotease (prtV). Arrows indicate the direction of transcription. Sizes are approximate.