TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Target |
|---|---|---|
| KanD-S1 | GGTTTCATTTGATGCTCGATGAG | Kmr gene |
| KanD-S2 | GATGTTGGACGAGTCGGAATCG | Kmr gene |
| KanD-S3 | CGCTACCTTTGCCATGTTTCAG | Kmr gene |
| KanD-S4 | CGAGCAAGACGTTTCCCGTTG | Kmr gene |
| MT3 | GCGCAATTAACCCTCACTAAAGGG | pBluescript |
| MT7 | GCGTAATACGACTCACTATAGGGC | pBluescript |
| EmpA-F1 | TACAAATTAATTCTCATC | empA |
| EmpA-R1 | TGGCAAGAAAAGACTAG | empA |
| EppF3 | CTGCAGTGATTTAGGGGTAGTCAATGAAGATAA | epp |
| EppR3 | CTGCAGGGTTAAGTGATCAATCGCTTTAATTC | epp |
| SDeppF | GCTAGGAGCTCGCTATACAGGCCCTAATGGAGA | epp |
| SDeppR | GCTAGTCTAGAAGCGTACTTTTTGTGCCTTGAA | epp |
| pNQ706-R | GCGTAACGGCAAAAGCACCGCCGGACATCA | pNQ705-1 |
| Epp(complement)F | GCTAGCTGCAGCATTAATCGAACGAGGTGTGA | epp |
| Epp(complement)R | GCTAGCTGCAGTTAGCGCAGCAGCCAGAAT | epp |
a
Restriction sites within primers are in boldface.