cAMP-CRP negatively regulates expression of genes involved in VPS biosynthesis and biofilm matrix protein production. β-Galactosidase assays of wild-type, ΔcyaA, and Δcrp strains harboring (A) vpsL-lacZ, (B) rbmA-lacZ, (C) rbmC-lacZ, and (D) bap1-lacZ fusion constructs were performed. The data are representative of at least two independent experiments. The error bars indicate standard deviations.