FIG. 1.

Absorption spectra of purified DevS. The spectra were recorded using 300 μg of DevS in 20 mM Tris-HCl buffer at RT. The DevS protein was purified in the absence of any reducing reagent in the buffer used during the purification procedures. (A) DevS was reduced by the addition of DTT to a final concentration of 10 mM and sparged with N₂ gas for 10 min to yield a deoxyferrous derivative of DevS (solid line). The deoxyferrous form of DevS was exposed to air to generate the oxyferrous form of DevS (dashed line). (B) The spectra of the purified DevS before (solid line) and after (dashed line) 10 mM KCN treatment.