FIG. 7.

The multiple sequence alignment of DevS of M. smegmatis with its homologs in M. tuberculosis. Amino acid sequences of DevS from M. smegmatis (Msm_DevS) and DosT (Mtb_DosT) and DevS (Mtb_DevS) from M. tuberculosis were aligned. Numbering was done using DevS of M. smegmatis. The arrows and coils above the aligned sequences indicate the elements of the secondary structure of the DevS GAF-B domain. Conserved residues are shown by the gray background. The putative transmembrane motifs, which were predicted with M. tuberculosis DevS, are denoted by thick lines below the alignment. The amino acid residues corresponding to those hydrogen bonded to a cyclic nucleotide in PDE2A and CyaB2 are indicated by the inverted triangles. Multiple alignment was performed using ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/) and visualized using ESPript (http://espript.ibcp.fr/ESPript/ESPript/).