Strains |
|
|
E. coli
|
|
|
DH5α |
(φ80dlacZΔM15) ΔlacU169 recZ1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1
|
19 |
BL21(DE3) |
F−ompT hsdSB(rB−mB−) dcm gal λ(DE3) |
Promega |
M. smegmatis mc2155 |
High-transformation-efficiency mutant of M. smegmatis ATCC 607 |
44 |
Plasmids |
|
|
pBS II KS+ |
Apr; lacPOZ′ |
Stratagene |
pNC |
Hygr; promoterless lacZ
|
Y. M. Kim, unpublished data |
pT7-7 |
Apr; T7 promoter, ribosome binding site, and translation start codon overlapping with NdeI site |
48 |
pT7SIHis |
pT7-7 with an insertion of a 1,727-kb NdeI-EcoRI fragment containing devS with six His codons before its stop codon |
This study |
pT7STHis |
pT7-T with an insertion of a 0.739-kb NdeI-EcoRI fragment containing the 3′ part of devS with six His codons before its stop codon |
This study |
pT7SIH150A |
pT7SIHIS in which the codon for H150 is displaced with GCT |
This study |
pT7HIS9A |
pT7-7 with an insertion of a 1.4-kb NdeI-PstI fragment containing prrB with nine His codons before its stop codon |
34 |
pNChspX |
pNC with an insertion of a 0.498-kb ClaI-XbaI fragment containing the hspX promoter region |
This study |
pGST-parallel1-GAFB |
pGST-parallel1 containing an NcoI-EcoRI fragment encoding the GAF-B domain (D232 to S373) |
B. S. Kang, unpublished data |