TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or genotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | (φ80dlacZΔM15) ΔlacU169 recZ1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 | 19 |
| BL21(DE3) | F−ompT hsdSB(rB−mB−) dcm gal λ(DE3) | Promega |
| M. smegmatis mc2155 | High-transformation-efficiency mutant of M. smegmatis ATCC 607 | 44 |
| Plasmids | ||
| pBS II KS+ | Apr; lacPOZ′ | Stratagene |
| pNC | Hygr; promoterless lacZ | Y. M. Kim, unpublished data |
| pT7-7 | Apr; T7 promoter, ribosome binding site, and translation start codon overlapping with NdeI site | 48 |
| pT7SIHis | pT7-7 with an insertion of a 1,727-kb NdeI-EcoRI fragment containing devS with six His codons before its stop codon | This study |
| pT7STHis | pT7-T with an insertion of a 0.739-kb NdeI-EcoRI fragment containing the 3′ part of devS with six His codons before its stop codon | This study |
| pT7SIH150A | pT7SIHIS in which the codon for H150 is displaced with GCT | This study |
| pT7HIS9A | pT7-7 with an insertion of a 1.4-kb NdeI-PstI fragment containing prrB with nine His codons before its stop codon | 34 |
| pNChspX | pNC with an insertion of a 0.498-kb ClaI-XbaI fragment containing the hspX promoter region | This study |
| pGST-parallel1-GAFB | pGST-parallel1 containing an NcoI-EcoRI fragment encoding the GAF-B domain (D232 to S373) | B. S. Kang, unpublished data |