FIG. 3.

Organization of transcriptional regulatory elements of the yiaK-S system. (A) The yiaK-S promoter sequence is numbered relative to the 5′ end determined in this study, which is indicated by an arrowhead labeled +1. The −10 and −35 promoter sequences of yiaK are underlined. The directions of the yiaJ and yiaK open reading frames (ORF) are indicated by dotted arrows. Predicted ATG start codons are indicated by bold type, and the corresponding ribosome binding sites (RBS) are also indicated. The IHF, cAMP-CRP, and YiaJ binding sites are indicated by shading. The inverted repeat of the YiaJ operator is indicated by asterisks. (B) Identification of yiaK-S 5′ end by sequencing across ligation sites of the 5′-RACE product, shown at the left site. The chromatogram shows the sequences at the ligation site of a typical cloned 5′-RACE product derived from a transcript obtained from K. pneumoniae ATCC 13882 grown on l-ascorbate. The arrow indicates the transcription initiation site.