TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10(Tcr)] | Stratagene |
| JA134 | ECL1 Lyx+ | 26 |
| JA184 | JA134 himA::cat | 12 |
| S17-1(λ-pir) | Tpr SmrrecA thi pro hsdR−M+RP4:2-Tc:Mu:Km Tn7 λpir | Biomedal |
| Klebsiella strains | ||
| K. pneumoniae ATCC 13882 | K. pneumoniae subsp. pneumoniae deposited as K. aerogenes | ATCC |
| K. pneumoniae ATCC 13883 | K. pneumoniae subsp. pneumoniae | ATCC |
| K. pneumoniae KC2653 | hutC515 dad-1 Δ[bla]-2 str-6 | 16 |
| K. oxytoca | Wild type | ATCC |
| K. pneumoniae C3 | O1:K66 | M. Regué |
| EB6193 | RP4-2 tet:Mu-1 Kan::Tn7 integrant leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 uidA (ΔMuI):pir+thi Spr/Smr | R. A. Bender |
| Plasmids | ||
| pBluescript | AprlacZ | Stratagene |
| pGEM-T | AprlacZYA | Promega |
| pMAL-C2x | AprmalE α-lacZ lacIq | Biolabs |
| pMAL-C2x-yiaJ | yiaJ in pMAL-C2x | This study |
| pMAL-C2x-ulaR | ulaR in pMAL-C2x | This study |
| pRS415 | AprlacZYA | 27 |
| pCB1583 | Apr Kmr oriR6K mobRP4 rpsL rbsA′ lacZ rbsK′ | R. A. Bender |
| pUTmini-Tn5 Km | Apr oriR6K mobRP4 tnp* mini-Tn5 Km | Biomedal |
| pUTmini-Tn5 Tc | Apr oriR6K mobRP4 tnp* mini-Tn5 Tc | Biomedal |
| pCAT19 | Apr Tn9-CAT(Cmr) | 8 |