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Expression of hilA in wild-type cells and cells in which dnaKJ is disrupted. Total RNA was prepared from strains χ3306 (dnaKJ+), CS2021 (ΔdnaKJ), and CS2501 (ΔdnaKJ/pdnaKJ+) grown in L broth to an OD600 of 0.5 at 30°C. The levels of hilA transcripts were measured by quantitative real-time RT-PCR and then normalized to 16S rRNA gene expression. The values represent the means and standard deviations of n-fold changes in comparison with the transcription level in χ3306. pdnaKJ+, pTKY608.
