FIG. 6.
Effect of dnaKJ disruption on expression of σ32 and lon in Salmonella cells. (A) Whole-cell extracts were prepared from strains χ3306 (dnaKJ+), CS2021 (ΔdnaKJ), and CS2501 (ΔdnaKJ/pdnaKJ+) grown in L broth to an OD600 of 1.0 at 30°C and separated on an SDS-10% polyacrylamide gel. The separated proteins were immunostained with anti-σ32 serum and anti-Lon serum. pdnaKJ+, pTKY608. (B) The expression levels of lacZ fusion to the lon promoter in cells harboring pTKY821 were assayed for β-galactosidase activity. The values represent the means and standard deviations of samples tested at least in triplicate. The strains used were CS3613 (dnaKJ+) and CS3614 (ΔdnaKJ).