Figure 2.

Characterization of Amph2 and its antiserum. (A) Amph2 is a 92-kDa protein present in the brain in the same molar ratio as Amph1. Total brain extract, extracts of COS cells transfected with either of the Amphs, and bacterially expressed protein were loaded on SDS-PAGE gels for comparison, followed by immunoblotting with each of the Amph antibodies. (B) Tissue distribution of Amph2 resembles that of Amph1. Brain extract (20 μg) and each of the other tissues (5 μg) were loaded on SDS-PAGE gels and immunoblotted with each antibody as indicated. A longer exposure of Amph2 reveals Amph2 reactivity in other tissues.