FIG. 5.

Transcription start points of phoP of C. acetobutylicum. The products of primer extension reactions using IRD800-labeled oligonucleotides were run on polyacrylamide gels alongside the corresponding sequencing reactions (T, G, C, A) generated with the same primer (A). (B) Locations of the identified transcription start points, PE1 (primer, phoP-neu-PE2), PE2 (phoP-neu-PE3), and PE3 (phoP-neu-PE3) are highlighted (gray circles) within the DNA sequence of the promoter region upstream of the phoP gene. The boldface ATG symbolizes the start codon of the phoP gene, and the arrow indicates its orientation; its dedicated ribosome binding site is underlined. Putative regulatory transcriptional elements of the transcription start points are boxed with a −10 or −35 (see text for details). Numbers in parentheses indicate their assignment to the corresponding transcription start points. Broken lines appear above possible regulatory repeats.