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Autophosphorylation of MBP-*PhoR and phosphate transfer to PhoP Strep-Tag. Ten μCi of [γ-32P]ATP was incubated with 2 μM MBP-*PhoR (lane 1) or with 2 μM MBP-*PhoR and 17 μM Strep-Tag-PhoP (lane 2) for 15 min at room temperature and was separated by SDS-PAGE (12.5%). Shown is an autoradiogram; relative signal intensities referring to the certain proteins are given below the lanes.
