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Maximum likelihood phylogenetic tree of sequenced Escherichia and Shigella strains based on MLST. The alignments were produced with ClustalW (14). Modeltest 3.5 (66) was used to determine the most appropriate evolutionary model. The tree was created with PAUP* (83). Nodes marked with circles have ≥70% neighbor-joining bootstrap support. Similar results were obtained for trees calculated by neighbor-joining and parsimony methods.
