FIG. 3.

Circular representation of pSMS35_130. pSMS35_130 is shown as composed of transfer, virulence, and resistance regions. From outside to inside: circles 1 and 2 (from different regions), strand-specific depiction of all CDS, color coded according to predicted functions in gray (replication), dark green (transfer and maintenance), brown/gold/red (transposition and recombination), light blue (APEC virulence), pink (colicin biosynthesis and immunity), and green (antibiotic resistance) (IS1 elements are depicted in gold, IS26 elements in red, and all other CDS in black); circles 3 to 6, NUCmer sequence comparisons (>90% nucleotide identity) with the E. coli plasmids p1658/97 (circle 3), pAPEC-O1-ColBM (circle 4), pAPEC-O2-ColV (circle 4), pAPEC-O2-R (circle 6), and pSC138 from S. enterica serovar Choleraesuis (circle 6) (sequence matches in the same orientation are shown in red and those in inverted orientation in blue); circle 7, laterally transferred CDS (black); circle 8, trinucleotide sequence composition. Abbreviations (clockwise, with gene names in italics): tra-trb (traABCDEFGHIJKLMNPQRSTUVWXY and trbABCDEHIJ), type IV secretion system-like conjugative transfer system; parB′, ParB-like partition protein; ssb, single-strand binding protein; sitABCD, iron/manganese transport system; ompT, outer membrane protease 7; colB′, truncated colicin-B biosynthesis and immunity proteins; colM, colicin-M biosynthesis and immunity proteins; cmlA, chloramphenicol transferase 2; dhfrV, dihydrofolate reductase type V; sul2, dihydropteroate synthase type 2; strAB, aminoglycoside resistance proteins A and B; blaT, beta-lactamase TEM; aadA, aminoglycoside adenylyltransferase; tetRA, tetracycline resistance repressor and resistance protein, class A; aph, aminoglycoside 3′-phosphotransferase.