FIG. 3.

DIC micrographs of spores with or without coat defects after digestion with lysozyme and DNase. Spores of strains PS533 (wild-type) (a), PS3328 (cotE) (b), PS4149 (gerE) (c), and PS4150 (cotE gerE) (d) were digested, and the material remaining after digestion was examined by DIC microscopy as described in Materials and Methods. The PS533 spores had been chemically decoated prior to lysozyme treatment, and the arrowheads (b) point to a few spores that were not digested with lysozyme. Bar, 20 μm (all images are at the same magnification).