FIG. 5.

l-Alanine (A) and dodecylamine (B) germination of spores with or without coat proteins. (A) Spores of various strains were heat shocked and germinated with l-alanine, and the OD₆₀₀ of cultures was monitored to assess spore germination, as described in Materials and Methods. The OD₆₀₀ falls ≥60% upon completion of wild-type spore germination, and this value was used to calculate the degree of spore germination at various time points. (B) Spores of various strains were incubated with dodecylamine, and DPA release was monitored to assess spore germination, as described in Materials and Methods. ○, PS533 (wild-type); •, PS3328 (cotE); ▵, PS4149 (gerE); ▴ PS4150 (cotE gerE).