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Vector maps. (A) pTET derived from pUV15TetORs (11). This vector contains a tetracycline-inducible promoter (tetO), a multiple cloning site (MCS), transcription terminators (T), a constitutively expressed tetracycline repressor cassette (puv15-tetR), a hygromycin resistance cassette (hygR), a mycobacterial replication origin (MYC ori), and an E. coli replication origin (ColE1 ori). (B) pGFP obtained by insertion of GFP with no stop codon.
