FIG. 4.

Changes in the intensity and stability of fluorescence when GFP was expressed in M. smegmatis with a C-terminal fusion to a 12-residue sequence from DesA3 (constructs 4B and 4C) or the corresponding sequence of the DesA3 homolog from M. smegmatis, Msmeg_1886 (constructs 4D and 4E). The fluorescence of GFP was measured 0, 1, 2, 4, 6, and 8 h after removal of anhydrotetracycline used to induce expression. A change of the C-terminal Ala residue to Asp increased the level of GFP fluorescence.