FIG. 10.

Accumulations of RNA1 mutants with nucleotide substitutions in the 3′ UTR of RNA1 (Fig. 3C) in BY-2 protoplasts. Inoculated protoplasts were incubated at 17°C for 18 h. Total RNA was extracted from protoplasts and used for Northern blotting with the 3′ UTR short probe. For other conditions, refer to the legend for Fig. 1.