FIG. 2.

SR1f is not a transcript but a degradation intermediate of RNA1. Time course analysis of the accumulations of viral replicase proteins (p27) and viral RNAs in BYL incubated with RNA1 and R1-p27fs. RNA1 or R1-p27fs (2 pmol) was incubated in 110 μl of BYL translation reaction mixture at 17°C. At different times after incubation, 10-μl aliquots were used for Northern and Western blotting, respectively.