FIG. 3.

Essential regions required for the generation of SR1f. (A) Schematic representation of deleted regions in the CP ORF and the 3′ UTR of RCNMV RNA1. The boldface lines indicate virus-derived sequences with the nucleotide numbers at the 5′ and 3′ ends. The bent lines indicate deleted regions. The Gs in the open boxes show the nucleotide residue in the 5′ end of SR1f. (B) Accumulation of small RNAs (SR1f) generated from RNA1 deletion mutants in BYL at 0.2 and 60 min after incubation. Genome RNAs and small RNAs (SR1f) were detected by RNA1/3′P. (C) Schematic representation of nucleotide substitution mutations in the 3′ UTR of RNA1. Italics in the open boxes show altered sequences. (D) Accumulation of small RNAs (SR1f) generated from RNA1 mutants in BYL at 0.2 and 60 min after incubation. Genome RNAs and small RNAs (SR1f) were detected by the 3′ UTR short probe.