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. 2008 Aug 13;82(20):10162–10174. doi: 10.1128/JVI.01027-08

FIG. 7.

FIG. 7.

Effects of SR1f on cap-dependent and cap-independent translation in BYL and BY-2 protoplasts. (A and B) SR1f trans inhibited cap-independent and cap-dependent translation in BYL. R1-5′-XbS (2.7 pmol) and pA60(+) (2.5 pmol) were incubated with the indicated amount of SR1f or SR1f/TE-Lm1 in 25 μl of BYL at 17°C for 120 min. The luciferase activity of R1-5′-XbS or pA60(+) with no competitor was defined as 100%. The error bars indicate standard deviations. (C and D) BY-2 protoplasts were inoculated with R1-5′-XbS (8 pmol) and pA60(+) (8 pmol) with an eightfold molar excess of SR1f or SR1f/TE-Lm1. The luciferase activities of R1-5′-XbS and pA60(+) without competitor were defined as 100%, respectively. The error bars indicate standard deviations.