FIG. 8.

SR1f trans inhibits negative-strand RNA synthesis of RNA1 and RNA2 in BYL. Accumulations of p27, positive-strand R1-m1, SR1f or SR1f/TE-Lm1, negative-strand R1-m1, positive-strand RNA2, and negative-strand RNA2 in BYL (25 μl) incubated with R1-m1, RNA2 (0.75 pmol), and a fivefold molar excess of SR1f or SR1f/TE-Lm1 at 17°C for 240 min (left panel). Total RNAs and proteins were extracted at 240 min after incubation and used for Northern and Western blotting, respectively. Quantification of the accumulations of p27 and negative-strand RNAs of R1-m1 and RNA2 was performed with the Image Gauge program (Fuji Photo Film, Tokyo, Japan) (right panel). Accumulations of p27, the negative-strand RNAs of R1-m1, and RNA2 in BYL incubated with R1-m1 and RNA2 were defined as 100%. The error bars indicate standard deviations.