FIG. 1.

Transcription map of B19V and probes used. (A) The B19V genome of the J35 virus (GenBank accession no. AY386330) is depicted, including the locations of the P6 promoter, the intron donors (D1 and D2) and acceptors (A1-1, A1-2, A2-1, and A2-2), the internal polyadenylation sites [(pA)p1 and (pA)p2], the distal polyadenylation site [(pA)d], and the ITRs. The four open reading frames (ORFs) are diagramed at the top of the figure. The different open reading frames that were used are shown with different shading patterns. The major transcript species and the proteins they encode are shown below the diagramed B19V genome (46). (B) The probes used for RPA in this study, i.e., probe 6, probe 8, and probe 10, are diagramed at the bottom, and their protected bands are shown with relative sizes and identities. Probe 6 has been described previously (46).