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. 2008 Aug 6;82(20):9951–9963. doi: 10.1128/JVI.01162-08

FIG. 6.

FIG. 6.

Replication of the B19V genome initiated by AAV5 ITRs resulted in significant increases in B19V RNAs polyadenylated at (pA)d. Plasmids pSKB19NS(−)Cap and p5TRB19NS(−)Cap were transfected into 293 cells with different combinations of the pHIVAV5Rep and pHelper plasmids, as indicated at the top of the figure. After 48 h, total RNAs were isolated and were protected by probe 8 (A), probe 6 (B), or probe 10 (C). All of the protected bands are designated as described in the legend to Fig. 3, and all of the quantifications were also performed as described in the legend to Fig. 3.