FIG. 9.

Splicing and polyadenylation at the (pA)p site compete for the processing of B19V pre-mRNAs. COS-7 cells were transfected with pSVOd-based (pSVO) or pBluescript-based (pSK) constructs, as indicated by SV40 Ori (+) or SV40 Ori (−) at the top. The variant B19V minigenomes of B19NS(−)CapΔ1300, B19ΔD1A1CapΔ1300, and B19P6Cap are diagramed in Fig. 7B. After 48 h, total RNAs were prepared and were protected by probe 8 (A), probe 6 (B), and probe 10 (C). All of the protected bands are designated as described in the legend to Fig. 3, and all of the quantifications were performed as described in the legend to Fig. 3.