FIG. 4.

IFN-β induction in MHV-infected brain-derived primary cell cultures. (A and B) Primary hippocampal neuron cultures were infected with 5 PFU/cell of RA59 or RJHM or 50 PFU/cell of Sindbis (Sin) virus or mock infected. At 24 h p.i., whole-cell RNA was isolated, and IFN-β mRNA (A) and mRNA7 (B) levels were measured by real-time qPCR. Primary astrocyte cultures (C and D) and primary mixed glial cultures (E and F) were infected with 1 PFU/cell of RA59, RJHM, or SeV or mock infected. At 24 h p.i., IFN-β mRNA (C and E) and mRNA7 (D and F) levels were measured by real-time qPCR. Real-time qPCR data are represented as the relative induction over mock-infected cells. Error bars represent the standard error of the mean of three replicates, and data are derived from one representative experiment of two.