FIG. 2.

Immunoblot analysis of primary sheep microglia following exposure to PrP^Sc (microglia^Sc) and unexposed microglia (microglia^C). Microglia were lysed, treated with proteinase K (PK) (+) or not treated with proteinase K (−), precipitated with phosphotungstic acid, and immunoblotted using the monoclonal anti-PrP antibody F99.97.6.1. (A) Comparison of prion immunoreactivity from sheep brain, microglia^Sc, and microglia^C. (B) Comparison of proteinase K-resistant glycoform patterns between Rov9^Sc and microglia^Sc. The positions of molecular mass standards (in kilodaltons) are indicated to the left of the immunoblots.