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Phenotypes of primary sheep brain cultures and transformed glial cell line
| Cells | Method | Markera
|
|||
|---|---|---|---|---|---|
| RCA-I | CD14 | CD68 | Nonspecific esterase | ||
| Primary | Immunocytochemistry | + | + | − | + |
| Flow cytometry | + | + | − | NA | |
| Transformed | Immunocytochemistry | + | + | + | + |
| Flow cytometry | + | + | + | NA | |
The presence (+) or absence (−) of markers is shown. The markers are Ricinus communis agglutinin-1 (RCA-I), CD14 (catalog no. MM61A; VMRD, Inc.), CD68 (EBM11; Dako), and nonspecific esterase. NA, not applicable.
