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. 2008 Jul 30;82(20):10032–10041. doi: 10.1128/JVI.01050-08

FIG. 4.

FIG. 4.

Subinhibitory concentrations of N36Mut(e,g) increase the t1/2 of the inhibitor-sensitive state. Viral infectivity as a function of time of addition of fully inhibitory concentrations of the C34 peptide (200 nM) (A), the neutralizing N-HR directed bF-3674 Fab (200 μg/ml) (B), and the neutralizing MPER-directed MAbs 2F5 and 4E10 (50 μg/ml) (C) in a synchronized HXB2-Env pseudotyped viral infectivity assay in the presence and absence of a subinhibitory (2 μM) concentration of N36Mut(e,g) is shown. The experimental data (averages from four to eight experiments, with error bars representing standard deviations) were fit to a sigmoidal curve (see Materials and Methods). The t1/2 values for the inhibitor-sensitive state are as follows: C34 at 37°C, 19.0 ± 2.3 min; C34 plus N36Mut(e,g) at 37°C, 42.6 ± 1.8 min; C34 at 30°C, 34.0 ± 5.5 min; C34 plus N36Mut(e,g) at 30°C, 62.9 ± 6.9 min; bF-3674 at 37°C, 21.5 ± 2.0 min; bF-3674 plus N36Mut(e,g) at 37°C, 27.8 ± 1.8 min; bF-3674 at 30°C, 30.9 ± 6.7 min; bF-3674 plus N36Mut(e,g) at 30°C, 51.3 ± 4.3 min; 2F5 and 4E10 at 37°C, 15.0 ± 1.8 and 15.9 ± 1.7 min (respectively); 2F5 and 4E10 plus N36Mut(e,g), 44.1 ± 2.3 and 57.9 ± 2.3 min (respectively).