FIG. 7.
N36Mut(e,g) and Fab bF-3674 display synergistic antiviral activity against primary B and C subtype HIV-1 isolates in the Env-pseudotyped virus neutralization assay. Dose-response curves for antiviral activity of N36Mut(e,g) alone (green), bF-3674 alone (red), and bF-3674 plus N36Mut(e,g) in two fixed combination ratios (blue) against Env-pseudotyped viruses from B (A and B) and C (C and D) clades are shown. The concentrations for the combination curves refer to the concentration of Fab-3674. The experimental data are fit by nonlinear least-squares minimization to a simple binding isotherm given by % infection = 100/(1 + [I[]/IC50), where [I] is the concentration of fusion inhibitor. IC50s, DRIs, and CIs are provided in Table 3.