FIG. 4.

Growth of R-LM39 and R-LM113 recombinants and of control viruses R-LM5 and R-LM13. (A to G) Replicate cultures of J (A), J-Nectin1 (B), J-HVEM (C), J-HER2 (D), SKOV3 (E), I-143 tk⁻ (F), and HEp-2 (G) cells were infected with R-LM5, R-LM13, R-LM39, or R-LM113 at 1 PFU/cell. Progeny virus was harvested at 3, 24, and 48 h after infection and titrated in SKOV3 cells. (H) Viral growth values at 48 h. (I) Infection of an array of cell lines with R-LM113. Monolayers were infected at 5 PFU/cell. Infection was quantified at 24 h as EGFP expression by means of a fluorometer. R.F.U., relative fluorescence units.