Table 3.
High-copy suppression of spo12Δ/spo12Δ and spo13Δ/spo13Δ strains
| Tetrads (%) | Dyads (%) | Sporulation (%) | |
|---|---|---|---|
| Plasmid (in spo12Δ/spo12Δ) | |||
| YEp13 | 0.3 ± 0.6 | 99.7 ± 0.6 | 44.8 ± 2.3 |
| pRS425 | 0.3 ± 0.7 | 99.7 ± 0.7 | 61.5 ± 14.9 |
| 21–36 (YEp13-SPO12) | 79.6 ± 2.8 | 20.4 ± 2.8 | 52.6 ± 7.1 |
| 47-32 (YEp13-SPO12) | 72.8 ± 2.8 | 27.2 ± 2.8 | 58.3 ± 8.3 |
| pRS425-SPO12 | 52.5 ± 2.4 | 47.8 ± 2.4 | 76.2 ± 1.1 |
| Analysis of SPO13 (in spo12Δ/spo12Δ) | |||
| 17-1 (YEp13-SPO13) | 9.1 ± 5.9 | 90.9 ± 5.9 | 45.9 ± 5.0 |
| 30-10 (YEp13-SPO13) | 17.7 ± 4.7 | 82.3 ± 4.7 | 47.4 ± 3.2 |
| pRS425-SPO13 | 7.1 ± 1.9 | 92.9 ± 1.9 | 70.1 ± 4.4 |
| Analysis of BNS1 (in spo12Δ/spo12Δ) | |||
| 32-1 (YEp13-BNS1) | 23.8 ± 5.5 | 76.2 ± 5.5 | 54.4 ± 7.3 |
| pRS425-BNS1 | 8.8 ± 3.3 | 91.2 ± 3.3 | 71.9 ± 5.7 |
| Plasmid (in spo13Δ/spo13Δ) | |||
| YEp13 | 5.3 ± 2.4 | 94.7 ± 2.4 | 50.1 ± 3.4 |
| pRS425 | 6.6 ± 3.0 | 93.4 ± 3.0 | 52.7 ± 7.6 |
| 17-1 (YEp13-SPO13) | 68.4 ± 4.4 | 31.6 ± 4.4 | 65.5 ± 5.1 |
| 30-10 (YEp13-SPO13) | 76.6 ± 4.2 | 23.4 ± 4.2 | 68.9 ± 7.1 |
| pRS425-SPO13 | 44.2 ± 4.5 | 55.8 ± 4.5 | 61.1 ± 8.5 |
| Analysis of SPO12 (in spo13Δ/spo13Δ) | |||
| 21-36 (YEp13-SPO12) | 23.0 ± 6.1 | 77.0 ± 6.1 | 53.9 ± 8.0 |
| 47-32 (YEp13-SPO12) | 18.3 ± 5.9 | 81.7 ± 5.9 | 51.3 ± 6.8 |
| pRS425-SPO12 | 11.4 ± 2.7 | 88.6 ± 2.7 | 54.3 ± 5.4 |
| Analysis of BNS1 (in spo13Δ/spo13Δ) | |||
| 32-1 (YEp13-BNS1) | 8.7 ± 5.7 | 91.3 ± 5.7 | 52.7 ± 2.7 |
| pRS425-BNS1 | 9.8 ± 6.4 | 90.2 ± 6.4 | 39.9 ± 6.0 |
Plasmids were transformed into an a/α spo12Δ/spo12Δ strain (YMG270) or an a/α spo13Δ/spo13Δ strain (YMG294). These strains were sporulated on plates, and 200 cells from each of four independently derived transformants per plasmid were analyzed for efficiency of sporulation and for asci. Tetrad values are the number of tetrads divided by the number of tetrads plus the number of dyads. Dyad values are the number of dyads divided by the number of tetrads plus the number of dyads. Sporulation values are the number of tetrads plus dyads divided by the total number of cells counted in each case (200).