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. 2008 Sep 18;9:424. doi: 10.1186/1471-2164-9-424

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Copyright © 2008 An et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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qPCR verification of the randomly selected bursicon-regulated genes identified by microarray. The ligated flies were injected with 0.5 μl r-bursicon heterodimer and the control flies received r-bursicon α protein only. See Methods for RNA extraction, PCR amplification, reverse transcription and mRNA quantification protocols. a. Genes regulated 1 h after r-bursicon injection. b. Genes regulated 3 h after r-bursicon injection. The data represent the mean ± SE of three biological samples. *: these genes were randomly selected for temporal transcriptional profile analysis in Fig. 5 and Fig. 6.