Figure 10.

Anchored nuclei formed in Nup93- complex–depleted Xenopus egg cytosol show greatly decreased staining with mAb414. Egg cytosol was immunodepleted with either anti-Nup93 antibodies (D₉₃) or nonspecific rabbit IgG (D_c). The rim stain of D_c nucleus (a) and the rim stain of a D₉₃ nucleus (b) at 1500× magnification are shown. The mAb414 staining of the D₉₃ nucleus is dimmer and patchy compared with the control. (c) Another D₉₃ nucleus at a higher-intensity integration, which is 4× the integration of a and b. (d) Same nucleus as in c is shown but image is focused on the surface of the nucleus.