Figure 4.

Localization of Nup93 in RLNES. (A) Cross-section of a single NE and selected examples of NPCs labeled with the polyclonal anti-Nup93 antibody conjugated to 8-nm colloidal gold by preembedding labeling. The rightmost examples (middle and bottom) are NPCs labeled with the mAbs QE5 and RL31 (anti-p62) conjugated to 8-nm colloidal gold, respectively. Cytoplasmic (c) and nuclear (n) sides of the NE are indicated. Bar, 100 nm. (B–D) Quantitative analysis of gold particles associated with the nuclear side of NPCs after preembedding labeling with 5 μg/ml anti-Nup93 antibody. (B) Sixty particles were scored and 20 μg/ml of anti-Nup93 antibody. (C) Fifty-five particles were scored after postembedding labeling with 1 μg/ml anti-Nup93 antibody. (D) Forty particles were scored.