Figure 7.

Nup93 is depleted from Xenopus egg cytosol by immunodepletion with anti-Nup93 antibodies. To determine the extent of depletion of Nup93 from the Nup93-depleted extract and membrane, different amounts of D_c cytosol were electrophoresed on an 8% polyacrylamide gel in lanes 1–6 (0.01, 0.02, 0.04, 0.1, 0.2, and 0.5 μl, respectively). D₉₃ extract was electrophoresed in lane 8 (1 μl). Examination indicates that the D₉₃ cytosol was depleted of more than 99% of Nup93 when compared with D_c in lane 2 (0.02 μl). Washed membranes (1 μl) contained a small percentage of Nup93, as shown in lane 9, estimated to be 0.2% of the total Nup93 normally present in the egg extract.