Figure 4.

Histological findings and immunocytochemical analysis of extracellular matrix at one week and four weeks after transplantation. The transplanted gelatin hydrogels are found in the corneal stroma in all groups. H&E staining reveals no mononuclear cell infiltration around gelatin hydrogels in all groups. The precursor/gelatin group shows more intense staining of laminin, type I collagen, type IV collagen, and vimentin in the transplanted gelatin than in the gelatin and fibroblast/gelatin groups one week after transplantation (A). These expressions in the precursor/gelatin group increase four weeks after transplantation (B). Scale bar=100 μm.