Table 2. Antibodies used in this study.
| Primary antibody | Laboratory* | Animal source | Dilution | Time of incubation | Time of DAB |
| CB | Sigma | Mouse | 1∶2500 | Overnight / 4°C | 2 min |
| SP | Medicorp | Rat | 1∶50 | Overnight / 4°C | 4 min |
| GAD | Millipore | Rabbit | 1∶1000 | Overnight / 4°C | 3 min |
| TH | Diasorin | Mouse | 1∶250 | Overnight / 4°C | 4 min |
| ENK | Medicorp | Mouse | 1∶50 | 48 h / 4°C | 5 min |
*
Sigma: St.Louis, MO, USA; Medicorp: Montreal, Canada; Millipore: Billerica, MA, USA; Diasorin Inc: Stillwater, MN, USA.
Abbreviations: CB, calbindin-D28k; DAB, 3,3′-diaminobenzidine tetrahydrochloride; ENK, enkephalin; GAD, glutamic acid decarboxilase; SP, substance P; TH, tyrosine hydroxylase.